What Are the 5 Stages of IVF?

by | Apr 8, 2023 | IVF

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What is IVF?

In Vitro Fertilization (IVF) is an assisted reproductive technology (ART) that helps couples conceive by combining the sperm and egg outside the body in a laboratory. Once the embryo is formed, it’s transferred back into the woman’s uterus, with the hope of implantation and a successful pregnancy.

Why Choose IVF?

IVF is often considered when other fertility treatments have been unsuccessful or when specific fertility issues are present, such as:

  • Blocked or damaged fallopian tubes
  • Male factor infertility
  • Endometriosis
  • Ovulation disorders
  • Unexplained infertility

Now that we know what IVF is and why it’s chosen, let’s explore the five stages of the IVF process.


The 5 Stages of IVF Are:

The 5 Stages of IVF

The 5 Stages of IVF

1. Ovulation Induction

During this initial stage, fertility medications are administered to stimulate the ovaries to produce multiple mature eggs. Regular monitoring through blood tests and ultrasounds ensures the proper development of follicles and eggs and determines the ideal time for egg retrieval.

2. Egg Retrieval

Once the eggs are mature, a minor surgical procedure is performed to extract them from the ovaries. The procedure involves the use of a thin needle, guided by ultrasound, to collect the eggs from each ovary.

3. Fertilization

In the laboratory, the retrieved eggs are combined with sperm, either from a partner or donor, to facilitate fertilization. In some cases, a technique called Intracytoplasmic Sperm Injection (ICSI) is used, where a single sperm is injected directly into the egg to increase the chances of fertilization.

4. Embryo Culture

The fertilized eggs, now embryos, are closely monitored for several days as they develop. The embryologist ensures the embryos are growing correctly and selects the best quality embryos for transfer into the uterus.

5. Embryo Transfer

The final stage involves transferring one or more embryos into the woman’s uterus using a thin catheter. If an embryo successfully implants into the uterine lining, pregnancy occurs. Any remaining high-quality embryos can be frozen for future use.

Also Read: IVF Procedure Step By Step

IVF Process: Step-by-Step Timeline

  1. Consultation and Testing (1-2 weeks): The IVF journey begins with an initial consultation and a series of diagnostic tests to evaluate fertility in both partners. These tests include blood work, semen analysis, and ultrasound examinations to develop a personalized treatment plan.
  1. Ovulation Induction (2-4 weeks): During this phase, fertility medications are administered to stimulate the ovaries to produce multiple mature eggs. Monitoring through blood tests and ultrasounds is done regularly to ensure proper follicle and egg development.
  1. Egg Retrieval (1 day)Once the eggs are mature, a minor surgical procedure is performed to collect them from the ovaries. The procedure typically takes 20-30 minutes and is performed under anesthesia.
  1. Fertilization and Embryo Culture (3-6 days): In the lab, the retrieved eggs are combined with sperm, either from a partner or donor, to facilitate fertilization. The resulting embryos are monitored for several days as they develop.
  1. Embryo Transfer (1 day): The final stage involves transferring one or more embryos into the woman’s uterus. If an embryo successfully implants into the uterine lining, pregnancy occurs. Any remaining high-quality embryos can be frozen for future use.
  1. Pregnancy Test (10-14 days post-transfer): A blood test is conducted to measure the levels of the pregnancy hormone, hCG, to determine if the IVF cycle was successful.


IVF Success Rate in India:

The success rate of IVF in India has been steadily improving, thanks to advancements in technology and increased expertise among fertility specialists. As of 2021, the average IVF success rate in India ranges from 30% to 50%, depending on factors like age, fertility issues, and the quality of the clinic.

Factors Influencing IVF Success Rates

  • Age of the woman: younger women have a higher chance of IVF success, as egg quality and quantity decline with age.
  • Cause of infertility: Some fertility issues, like severe male factor infertility or uterine abnormalities, may lower the chances of IVF success.
  • Quality of the fertility clinic: Choosing a reputable clinic with skilled professionals and state-of-the-art technology is crucial for improving the chances of a successful IVF cycle.
  • Number of previous cycles: Success rates may decline with multiple IVF cycles, but cumulative success rates increase with each subsequent cycle.


IVF Cost in India:

The cost of IVF in India varies depending on factors such as the clinic’s location, the expertise of the fertility specialist, and the specific treatments required. On average, an IVF cycle in India costs between INR 1.5 to 3 lakhs. This cost typically includes:

  • Consultation fees
  • Diagnostic tests
  • Medications
  • IVF procedure
  • Laboratory charges

It’s important to note that additional costs may be incurred if specialized techniques such as ICSI, donor sperm/eggs, or surrogacy are required.


How Many Injections for IVF Treatment?

The number of injections required for IVF treatment depends on the specific fertility medications prescribed and the individual’s response to the drugs. On average, patients may require 1-3 injections per day for a period of 10-12 days during the ovulation induction phase.


How Many Days After Egg Retrieval Is Embryo Transfer?

Embryo transfer typically takes place 3-6 days after egg retrieval. The decision on when to transfer the embryo depends on the quality and development of the embryos, as well as the individual’s medical history.

Day 3 vs. Day 5 Transfer

A Day 3 transfer involves placing the embryo into the uterus three days after fertilization, while a Day 5 transfer, also known as a blastocyst transfer, involves transferring the embryo five days after fertilization. Day 5 transfers are becoming more common, as they allow for better embryo selection and may improve the chances of successful implantation.

Is IVF Painful?

While individual experiences vary, most patients report that IVF is not overwhelmingly painful. However, there are certain aspects of the process that may cause discomfort or mild pain.



Q: What is the most critical step in IVF?

A: The most critical step in IVF is the fertilization of the egg with the sperm, which usually takes place in a laboratory dish.

Q: How long is the IVF process from start to finish?

A: The IVF process can take several weeks to complete, from the start of ovarian stimulation to embryo transfer. The duration can vary depending on individual circumstances and medical conditions.

Q: Which cycle in IVF is successful?

A: Success rates of IVF cycles can vary depending on various factors, such as age, medical history, and fertility issues. In general, the first cycle has a lower success rate than subsequent cycles.

Q: What are the signs of successful IVF?

A: Signs of successful IVF include pregnancy symptoms such as missed periods, positive pregnancy tests, and ultrasound confirmation of fetal development.

Q: What is high risk of IVF?

A: IVF carries some risks such as multiple pregnancies, ovarian hyperstimulation syndrome, ectopic pregnancy, and birth defects. However, the overall risk is generally low.

Q: Is IVF successful in the first try?

A: The success rate of IVF varies depending on several factors, including the age of the woman, the cause of infertility, and the quality of the eggs and sperm. Although some people may get pregnant after the first cycle, it may take multiple attempts for others.

Q: How many injections are given in IVF?

A: The number of injections given during IVF varies depending on the protocol, but typically, several injections are given over the course of several weeks to stimulate the ovaries and support the growth and maturation of multiple eggs.

Q: Can you travel during IVF?

A: Travel during IVF may be possible, but it may require careful planning and coordination with the medical team. Factors such as medication schedules, embryo transfer dates, and travel restrictions should be considered.

Q: How many days of injections for IVF?

A: The duration of injections during IVF can vary depending on the protocol and individual circumstances. Typically, injections are given for about 8-14 days, but this can vary depending on the response to medication and the number of mature eggs required for retrieval.

Q: Which month is best for IVF?

A: There is no specific month that is best for IVF. However, it is recommended that IVF be scheduled around the menstrual cycle to optimize the timing of egg retrieval and embryo transfer.


Appointment By Prior Booking only

    Dr Mona Dahiya

    Dr Mona Dahiya

    IVF Specialist & Consultant

    Dr Mona Dahiya has performed over 5,000+ IVF cycles and is considered a global expert in IVF, ICSI, IUI and male fertility treatment. She is an eminent writer on Infertility Treatment and has over 100 Publications in both International and National Journals. Dr Mona Dahiya has immensely contributed to the field of infertility through her Research and articles.

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