IVF Procedure Step By Step: IVF Process Start to Finish

by | Feb 18, 2023 | IVF Treatment

What is IVF?

In vitro fertilization (IVF) is a complex process that involves several steps. This includes ovarian stimulation, egg retrieval, fertilization in the laboratory, embryo culture, and finally, embryo transfer. The success of the ivf procedure depends on many factors, including the quality of the eggs and sperm, the expertise of the fertility clinic and healthcare providers, and the patient’s age and overall health. In this article, we will explore IVF Treatment Procedure Step By Step in detail, including the preparation, procedures, and post-treatment care. Understanding the IVF procedure step by step can help couples make informed decisions about fertility treatment and increase their chances of success.


What do you do to prepare for IVF Process?

  • Medical evaluation: Before starting IVF treatment, your doctor will evaluate your medical history and perform a physical examination. They may also recommend additional tests to check for any underlying medical conditions that may affect the success of the treatment.
  • Medications: Your doctor will likely prescribe medications to regulate your menstrual cycle and stimulate the growth of multiple eggs in your ovaries. You will need to follow the medication schedule carefully and attend all scheduled appointments.
  • Lifestyle changes: You may need to make some lifestyle changes to optimize your chances of success. This may include maintaining a healthy weight, reducing alcohol and caffeine consumption, and quitting smoking.
  • Emotional support: IVF treatment can be emotionally challenging, so it is important to have a strong support system in place. This may include talking to a therapist or joining a support group.
  • Financial planning: IVF treatment can be expensive, so it is important to plan for the costs in advance. You may need to explore insurance coverage, payment plans, or other financial options.

By following these IVF treatment process step by step you can maximize your chances of success. It is important to work closely with your doctor and care team throughout the process to ensure the best possible outcome.

Also Read: How IVF Treatment is Done


Here is IVF Process Start to Finish:

  • Step 1: Ovarian Stimulation
  • Step 2: Egg Retrieval
  • Step 3: Sperm Collection
  • Step 4: Fertilization
  • Step 5: Embryo Culture
  • Step 6: Embryo Transfer
  • Step 7: Implantation
  • Step 8: Pregnancy Test
  • Step 9: Follow Up Visit & Tests
  • Step 10: Cryopreservation

In vitro fertilization (IVF) is a process in which an egg is fertilized with sperm outside the body in a laboratory setting. This process is typically used by couples who have been unable to conceive naturally or by those who have a history of genetic disorders.

IVF Process Step By Step

IVF Process Step By Step

IVF Procedure Step By Step:

Step 1: Ovarian Stimulation

Ovarian stimulation is a critical component of in vitro fertilization (IVF) and involves the use of medications to stimulate the growth of multiple follicles in the ovaries. This process is crucial to the success of IVF as it allows for the retrieval of multiple mature eggs, increasing the chances of a successful fertilization and pregnancy. During ovarian stimulation, women typically receive a series of hormone injections over a period of several weeks. These medications, which typically include follicle-stimulating hormone (FSH) and luteinizing hormone (LH), work to increase the number of follicles in the ovaries and promote the growth and maturation of the eggs within them.

The progress of the ovarian stimulation process is monitored closely through regular ultrasounds and blood tests. These tests help to determine the optimal timing for egg retrieval, which is typically scheduled when the follicles have reached a certain size and the hormone levels are appropriate. While ovarian stimulation can increase the chances of success in IVF, it also carries some risks, such as the potential for ovarian hyperstimulation syndrome (OHSS) and multiple pregnancies. As a result, it is important for patients to work closely with their medical team to develop an individualized treatment plan that takes into account their specific medical history and goals for IVF.

Step 2: Egg Retrieval

Egg retrieval is a critical step in the in vitro fertilization (IVF) process, as it is the procedure by which mature eggs are collected from the woman’s ovaries. This minimally invasive surgical procedure is typically performed under mild sedation or general anaesthesia to minimize any discomfort for the patient. During the egg retrieval process, a small needle is inserted through the vaginal wall and into each ovary. The needle is guided using ultrasound imaging to ensure accurate placement. Once the needle reaches the follicles in the ovaries, suction is used to extract the follicular fluid, which contains the mature eggs.

After the eggs have been collected, they are immediately taken to the laboratory for fertilization using either conventional IVF or intracytoplasmic sperm injection (ICSI). The egg retrieval procedure typically takes around 20-30 minutes and most patients can return home the same day. While egg retrieval is generally a safe and routine procedure, there are some potential risks, such as bleeding, infection, and damage to the surrounding organs. Patients are carefully monitored before and after the procedure to minimize any risks and ensure a successful IVF cycle.

Step 3: Sperm Collection

There are several ways to collect sperm for use in IVF, including masturbation, surgical extraction, and testicular sperm extraction. Masturbation is the most common method of sperm collection for IVF. The man is provided with a private room where he can produce a semen sample by masturbating into a sterile cup. The sample is then analysed and processed to select the healthiest and most active sperm for fertilization.

In cases where there is a problem with sperm production or ejaculation, surgical extraction may be necessary. This can involve retrieving sperm directly from the testicles or epididymis using a needle or small incision. Regardless of the method used, the collected sperm must be carefully handled and processed to maximize the chances of successful fertilization. The sperm are washed and concentrated to remove any impurities and ensure the highest possible concentration of healthy sperm for use in IVF.

Step 4: Fertilization

The fertilization process in in vitro fertilization (IVF) involves the combination of eggs and sperm outside of the body in a laboratory setting. Once sperm have been collected, they are placed in a dish with the eggs and allowed to fertilize naturally or through a process called intracytoplasmic sperm injection (ICSI).

ICSI is used in cases where there are sperm-related fertility issues, such as low sperm count or poor sperm motility. In ICSI, a single sperm is injected directly into the egg using a tiny needle, increasing the chances of successful fertilization.

Step 5: Embryo Culture

Embryo culture is the process of growing and developing embryos in a laboratory after they have been fertilized in vitro. In IVF, embryos are typically cultured for two to six days before being transferred to the uterus or frozen for future use. During this time, the embryos are carefully monitored for signs of proper development and quality. The laboratory environment is designed to mimic the conditions of the human body to ensure the best possible chances of embryo growth and development. Embryo culture is a critical step in the IVF process and requires skilled technicians and advanced technology to maximize the chances of a successful pregnancy.

Step 6: Embryo Transfer

Embryo transfer is the process of placing one or more embryos into the uterus in order to achieve pregnancy in IVF. The transfer typically takes place two to six days after fertilization and embryonic development, depending on the specific circumstances of the case. The procedure is typically performed under ultrasound guidance and involves inserting a thin catheter through the cervix and into the uterus, where the embryos are gently placed. After the transfer, the patient may be advised to rest for a short period of time before resuming normal activities. The success of the transfer depends on many factors, including the quality of the embryos and the receptiveness of the uterus.

Step 7: Implantation

Implantation is a crucial step in the in vitro fertilization (IVF) process, where the fertilized egg or embryo is placed in the uterus to establish a pregnancy. In IVF, the embryo can be transferred to the uterus at different stages of development, ranging from day 2 to day 6 after fertilization. The implantation process is facilitated by hormone treatments, which help prepare the lining of the uterus for implantation. The success of implantation depends on several factors, including the quality of the embryo, the receptiveness of the uterus, and the timing of the transfer. Close monitoring and follow-up care can help ensure a successful pregnancy after implantation in the IVF process.

Also Read: IVF Babies: 7 Facts You Need to Know

Step 8: Pregnancy Test

A pregnancy test after IVF is an important step to confirm whether the IVF procedure was successful. Typically, a blood test called a beta hCG test is used to detect pregnancy. This test measures the levels of human chorionic gonadotropin (hCG), a hormone produced by the placenta after implantation. It is usually conducted 9-12 days after the embryo transfer. However, home pregnancy tests can also be used, but they may not provide reliable results as early as the beta hCG test. A positive pregnancy test after IVF is a cause for celebration, but it is important to continue monitoring the pregnancy closely to ensure a successful outcome.

Step 9: Follow Up Visit & Tests

After a successful IVF pregnancy, follow-up visits and tests are important to ensure the health of the mother and baby. These appointments typically occur in the first trimester and involve ultrasound scans to confirm the pregnancy, assess fetal growth and check for any abnormalities. Blood tests are also performed to check hormone levels and screen for genetic disorders. Further appointments are scheduled throughout the pregnancy to monitor the baby’s development and ensure the mother’s health. Women who conceive through IVF may have a higher risk of certain pregnancy complications, such as gestational diabetes and pre-eclampsia, and may require additional monitoring. Close follow-up care and communication with a healthcare provider can help ensure a healthy pregnancy and delivery.

Step 10: Cryopreservation

Cryopreservation, also known as embryo freezing, is a technique used in IVF to preserve embryos for future use. This is done by cooling the embryos to very low temperatures, typically -196°C, using liquid nitrogen. The process of cryopreservation allows couples to store excess embryos that are not transferred during an IVF cycle, providing them with an opportunity to try for a pregnancy at a later time. Cryopreservation is also beneficial for women who may need to delay their pregnancy due to medical reasons or personal choices.

The success rate of cryopreservation has improved significantly over the years, and frozen embryos have been shown to have similar pregnancy rates to fresh embryos. In addition, cryopreservation has also reduced the risk of multiple pregnancies and ovarian hyperstimulation syndrome, as fewer embryos are transferred during IVF cycles. Cryopreservation also has the potential to increase the success rates of IVF by allowing for additional attempts with frozen embryos. However, the decision to freeze embryos should be made in consultation with a fertility specialist, as not all embryos are suitable for cryopreservation.

IVF Process Start to Finish is a complex process that involves multiple steps and careful monitoring. While it can be a challenging and emotional journey for couples, it has provided many people with the opportunity to start families that would not have been possible otherwise. With advancements in technology and medicine, the success rates for IVF continue to improve, offering hope for couples who are struggling with infertility.

Also Read IVF Process/Procedure

IVF Risks/Side Effects:

  • Multiple pregnancies: One of the main risks of IVF is the potential for multiple pregnancies, which can increase the risk of complications for both the mother and babies.
  • Ovarian hyperstimulation syndrome (OHSS): Ovarian stimulation medications can sometimes cause the ovaries to become swollen and painful, a condition known as OHSS.
  • Ectopic pregnancy: IVF increases the risk of ectopic pregnancy, which occurs when a fertilized egg implants outside of the uterus.
  • Emotional and financial stress: IVF can be an emotionally and financially stressful process, particularly if multiple cycles are needed.


IVF Benefits:

  • Increased chance of pregnancy: For couples who have struggled with infertility, IVF can significantly increase the chances of a successful pregnancy.
  • Genetic testing: IVF allows for genetic testing of embryos before transfer, which can help identify and prevent certain genetic disorders.
  • Donor eggs or sperm: IVF can also make it possible to use donor eggs or sperm, allowing individuals or couples with genetic disorders or other fertility issues to conceive.


What to Expect During IVF:

During the IVF procedure, you can expect some mild discomfort, particularly during the egg retrieval and embryo transfer procedures. However, the procedure is generally well-tolerated, and most people can resume normal activities within a few days.


What to Expect After IVF:

After the embryo transfer, you will need to rest for a few days and avoid any strenuous physical activity. Around two weeks after the transfer, you will return to your fertility clinic for a pregnancy test to determine if the IVF was successful.

Also Read: IVF Process Step By Step In Hindi



Q: Is IVF Procedure Painful?

A: IVF procedure may cause some discomfort, but it should not be extremely painful. Women may experience cramping or mild pain during the egg retrieval process.

Q: How many rounds of IVF does it take to get pregnant?

A: The number of rounds of IVF needed to achieve pregnancy varies depending on individual circumstances. Some women may get pregnant after just one cycle of IVF, while others may require multiple cycles.

Q: IVF Treatment Cost in India?

A: The IVF treatment cost in India varies depending on the clinic and the type of IVF procedure. On average, the cost of a single IVF cycle in India can range from Rs. 1,30,000 to Rs. 2,50,000.

Q: How many injections for IVF treatment Procedure?

A: The number of injections required during an IVF cycle depends on the individual’s fertility situation and the specific IVF protocol being used. Typically, women need to take injections for 8-14 days.

Q: How effective is IVF in getting pregnant?

A: The effectiveness of IVF in achieving pregnancy varies depending on factors such as age, fertility history, and the specific circumstances of the individual. On average, the success rate of IVF in getting pregnant is around 30% per cycle.

Q: How long does it take to know you are pregnant after IVF?

A: Women can usually take a pregnancy test around two weeks after the embryo transfer procedure to determine if they are pregnant.

Q: How soon after a failed IVF procedure can you try again?

A: The amount of time between IVF cycles depends on individual circumstances, but it is typically recommended to wait at least one menstrual cycle before trying again.

Q: Can you select the gender during IVF?

A: Gender selection during IVF is possible through a process called preimplantation genetic testing (PGT), which can identify the sex of embryos before they are transferred.

Q: Is there anything I can do to increase my chances of pregnancy with IVF?

A: Some things that may increase the chances of pregnancy with IVF include maintaining a healthy weight, reducing stress, and following the recommended protocol and medication schedule.

Q: What is the success rate of IVF by age?

A: The success rate of IVF decreases as women age. For women under 35, the success rate is around 40%, while for women over 40, it drops to around 4-5%.

Q: Why do IVF Treatment Procedure Step By Step fail?

A: IVF treatments can fail for a variety of reasons, including low-quality embryos, implantation failure, hormonal imbalances, and underlying medical conditions.

Q: Can IVF cause birth defects?

A: Studies have shown that the risk of birth defects with IVF is only slightly higher than the risk for natural pregnancies. However, there is still some risk associated with the procedure.

Q: What is the best age to get IVF?

A: The best age to get IVF depends on individual circumstances, but generally, the chances of success are highest for women under 35.

Q: Should you freeze embryos during IVF Treatment Process?

A: Freezing embryos during IVF treatment can provide a backup in case the initial cycle is unsuccessful, and may also be helpful for women who want to delay pregnancy until a later time.

Q: How many eggs are needed for IVF?

A: The number of eggs needed for IVF varies depending on the individual’s fertility situation and the specific IVF protocol being used. On average, around 10-15 eggs are retrieved per IVF cycle.

Q: Are IVF pregnancies high risk?

A: IVF pregnancies are considered to be higher risk than natural pregnancies, but the extent of the risk depends on individual circumstances. Women who undergo IVF may have a slightly higher risk of complications such as preterm birth, low birth weight, and preeclampsia.

Q: What are the most common side effects of IVF?

A: The most common side effects of IVF include bloating, cramping, mood swings, and fatigue. Some women may also experience hot flashes and headaches.

Q: What is assisted hatching in IVF?

A: Assisted hatching is a technique used in IVF to help embryos hatch from their outer layer before they are implanted in the uterus. This can increase the probability of successful implantation and pregnancy.

Q: What medications are used for IVF?

A: There are several medications used during IVF, including gonadotropins to stimulate the ovaries to produce more eggs, as well as medications to prevent premature ovulation and prepare the uterus for implantation.

Q: How do IVF injections work?

A: IVF injections are used to stimulate the ovaries to produce more eggs than they would during a typical menstrual cycle. The injections contain hormones that stimulate the follicles in the ovaries to grow and mature, resulting in the production of multiple eggs.

Q: How long is the IVF Treatment Process from beginning to end?

A: The length of the IVF process can vary, but typically it takes around 4-6 weeks from the start of ovarian stimulation to the embryo transfer. This may be longer if additional procedures such as embryo freezing or genetic testing are involved.

Q: What are some reasons someone might consider IVF?

A: IVF is often recommended for people who have been trying to conceive for an extended period of time without success, or for people who have specific medical conditions that make it difficult to conceive naturally. Some common reasons someone might consider IVF include:

  • Blocked or damaged fallopian tubes
  • Low sperm count or poor sperm motility
  • Endometriosis
  • Unexplained infertility
  • Advanced maternal age
  • Genetic disorders
  • Recurrent miscarriages

Q: How do I know if I am a good candidate for IVF?

A: If you are struggling to conceive and have not had success with other fertility treatments, it may be worth considering IVF. Your doctor can help you determine if you are a good candidate based on factors such as your age, medical history, and fertility test results. Some factors that may affect your eligibility for IVF include:

  • Age: IVF success rates decrease with age, so people over 35 may have a lower chance of success.
  • Ovarian reserve: Your doctor will evaluate your ovarian reserve, or the number and quality of eggs remaining in your ovaries.
  • Sperm quality: Your partner’s sperm will also be evaluated to determine if IVF is a viable option.
  • General health: Your doctor will evaluate your overall health and medical history to ensure that IVF is safe for you.

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    Dr Mona Dahiya

    Dr Mona Dahiya

    IVF Specialist & Consultant

    Dr Mona Dahiya has performed over 10,000+ IVF cycles and is considered a global expert in IVF, ICSI, IUI and male fertility treatment. She is an eminent writer on Infertility Treatment and has over 100 Publications in both International and National Journals. Dr Mona Dahiya has immensely contributed to the field of infertility through her Research and articles.

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