Pre-IVF Tests: What Tests Are Done Before IVF?

by | Oct 5, 2023 | IVF

What is IVF?

IVF, or In Vitro Fertilization, is a complex assisted reproductive technology (ART) that offers hope to individuals or couples who are unable to conceive naturally. The process begins with the stimulation of the ovaries to produce multiple eggs, which are then retrieved through a minor surgical procedure. These eggs are subsequently combined with sperm in a laboratory setting, where fertilization occurs. Once fertilized, the resulting embryos are cultured for a few days to assess their quality. Finally, one or more healthy embryos are carefully selected and transferred into the woman’s uterus, with the hope that they will implant and result in a successful pregnancy.


Pre-IVF Tests: What Tests Are Done Before IVF?

Before initiating an IVF cycle, a series of pre-IVF tests are performed to determine the underlying causes of infertility and to ensure that the patient is physically and emotionally prepared for the procedure. These tests are crucial in tailoring the IVF treatment plan to the specific needs of the individual or couple. Here are some key pre-IVF tests:

1. Ovarian Reserve Testing:

Ovarian reserve testing assesses the quantity and quality of a woman’s eggs. This includes blood tests to measure hormone levels like Anti-Müllerian Hormone (AMH) and Follicle-Stimulating Hormone (FSH). A transvaginal ultrasound may also be performed to count the number of follicles in the ovaries.

2. Semen Analysis:

A semen analysis evaluates the quality and quantity of sperm in a male partner’s semen. This test assesses sperm count, motility, and morphology to determine if there are any issues with sperm that may affect fertilization.

3. Hysterosalpingography (HSG):

HSG is a radiologic procedure that checks for any blockages or abnormalities in the woman’s fallopian tubes and uterus. It involves injecting a contrast dye into the reproductive organs and taking X-ray images to visualize the structures.

4. Infectious Disease Screening:

Both partners may undergo tests for infectious diseases like HIV, hepatitis B, and hepatitis C. This is done to minimize the risk of transmitting infections to the embryo or the partner during the IVF process.

5. Genetic Testing:

Genetic tests such as karyotyping or carrier screening may be recommended to identify any genetic abnormalities that could impact the health of the offspring.

6. Mock Embryo Transfer:

A mock embryo transfer is a rehearsal of the embryo transfer procedure. It helps the fertility specialist determine the best approach for transferring embryos and ensures a smoother process on the actual transfer day.

7. Psychological Evaluation:

Infertility and IVF can be emotionally challenging. Psychological assessments or counseling may be offered to help individuals and couples cope with the stress and emotional aspects of IVF treatment.

8. Medical Evaluation:

A comprehensive medical evaluation includes a physical examination and review of medical history to identify any underlying health issues that may affect fertility or the success of the IVF procedure.


Blood Tests Before IVF Transfer in Males and Females

Before undergoing an IVF (In Vitro Fertilization) transfer, both males and females may need specific blood tests to assess their reproductive health.

Female Blood Tests Before IVF:

  1. Hormone Levels: Evaluating hormone levels is crucial for females. This includes testing for Follicle-Stimulating Hormone (FSH), Luteinizing Hormone (LH), Estradiol, and Anti-Müllerian Hormone (AMH). These hormones provide insights into ovarian function, egg quality, and ovarian reserve.
  2. Thyroid Function: Thyroid hormones, such as TSH (Thyroid-Stimulating Hormone) and free T4, are checked to ensure that thyroid function is within the optimal range. Thyroid imbalances can affect fertility.
  3. Prolactin: Elevated levels of prolactin can interfere with ovulation and menstruation, so this hormone is measured to rule out any issues.
  4. Infectious Disease Screening: Females undergo tests for infectious diseases like HIV, hepatitis B, and hepatitis C to prevent the transmission of infections to the embryo or partner during the IVF process.

Male Blood Tests Before IVF:

  1. Semen Analysis: Semen analysis is the primary blood test for males before IVF. It assesses sperm count, motility, morphology, and other sperm parameters. These factors influence fertilization success.
  2. Hormone Levels: Hormones like Testosterone, LH, and FSH are checked to evaluate the male reproductive system’s function. Hormone imbalances can contribute to male infertility.
  3. Infectious Disease Screening: As with females, males also undergo screening for infectious diseases to ensure the safety of the IVF process.


What Uterus Test  Are Done Before IVF Transfer?

In addition to blood tests, evaluating the condition of the uterus is critical for the success of IVF. Several tests can be conducted to assess the uterus:

  1. Hysterosalpingography (HSG): HSG is an X-ray procedure that examines the uterine cavity and fallopian tubes. It helps detect any abnormalities, such as uterine fibroids, polyps, or blockages in the fallopian tubes.
  2. Transvaginal Ultrasound: This ultrasound provides detailed images of the uterus, allowing doctors to look for structural issues like fibroids or polyps.
  3. Sonohysterogram: A saline infusion sonogram involves injecting saline into the uterus and using ultrasound to visualize the uterine cavity. This test can reveal abnormalities that may affect embryo implantation.
  4. Hysteroscopy: In some cases, a hysteroscopy is performed to directly examine the uterine lining. It can diagnose and potentially treat conditions like polyps or adhesions.


How Do I Prepare My Body For IVF?

Preparing the body for IVF involves several steps to optimize the chances of a successful pregnancy:

  1. Lifestyle Adjustments:
  • Maintain a healthy diet rich in nutrients.
  • Engage in regular exercise to promote overall well-being.
  • Manage stress through relaxation techniques, meditation, or counseling.
  1. Medication and Hormone Regulation:
  • Follow the prescribed medications and hormone treatments as advised by your fertility specialist.
  • Monitor and regulate any underlying medical conditions, such as diabetes or thyroid disorders.
  1. Avoid Harmful Substances:
  • Refrain from smoking, alcohol, and recreational drug use.
  • Minimize caffeine intake.
  1. Vitamin and Supplement Usage:
  • Consult with your healthcare provider about appropriate prenatal vitamins and supplements.
  1. Emotional Support:
  • Seek emotional support from counselors or support groups, as the IVF process can be emotionally challenging.
  1. Follow Pre-IVF Testing Recommendations:
  • Ensure that you complete all recommended pre-IVF tests and address any identified issues.


How Many Injections Are Given In IVF?

The number of injections given during an IVF cycle varies depending on the individual’s specific treatment plan. IVF typically involves the administration of hormonal medications via injections to stimulate the ovaries and promote egg development. The most common injections include:

  1. Follicle-stimulating hormone (FSH) Injections: These stimulate the growth of multiple follicles in the ovaries, each containing an egg.
  2. Luteinizing Hormone (LH) Injections: In some cases, LH injections may be used alongside FSH to support follicle development.
  3. Human Chorionic Gonadotropin (hCG) Trigger Shot: This injection is administered to induce the final maturation of the eggs and prepare them for retrieval.
  4. Progesterone Injections or Suppositories: After egg retrieval, progesterone injections or suppositories may be prescribed to prepare the uterine lining for embryo transfer and support early pregnancy.

The specific regimen and duration of injections are determined by the fertility specialist based on the patient’s individual needs and response to treatment.


What Hormone Is Tested For IVF?

Hormone testing is a critical aspect of IVF, both before and during the treatment cycle. Some key hormones monitored during IVF include:

  1. Follicle-Stimulating Hormone (FSH): FSH levels are assessed to determine the ovarian reserve and the response to ovarian stimulation medications.
  2. Luteinizing Hormone (LH): LH levels are measured to ensure proper ovulation induction.
  3. Estradiol: Estradiol levels are monitored to track the growth and maturation of ovarian follicles.
  4. Progesterone: Progesterone levels are evaluated to assess the readiness of the uterine lining for embryo transfer.
  5. Human Chorionic Gonadotropin (hCG): hCG levels are checked following the trigger shot to confirm the maturation of eggs and the timing of egg retrieval.
  6. Anti-Müllerian Hormone (AMH): AMH levels provide information about ovarian reserve and potential response to ovarian stimulation.

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    Dr Mona Dahiya

    Dr Mona Dahiya

    IVF Specialist & Consultant

    Dr Mona Dahiya has performed over 5,000+ IVF cycles and is considered a global expert in IVF, ICSI, IUI and male fertility treatment. She is an eminent writer on Infertility Treatment and has over 100 Publications in both International and National Journals. Dr Mona Dahiya has immensely contributed to the field of infertility through her Research and articles.

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